一、課程簡介:

課程對象(Class audience)：碩士班1, 2 年級(Master students)
上課時間(Class time)：週三3, 4 節及中午上課(Wednesday, 3, 4, and noon)
學分(credit)：選修3學分(3 credits)
課程簡介(Course objective)：
To facilitate students with the knowledge of environmental biotechnology as well as the techniques used in the field of environmental biotechnology.

二、先修課程(Prerequisite):
限修過環境生物技術概論或生物化學相關科目或經任課老師同意。
(Limited to students who had taken Introduction to Environmental Biotechnology, Biochemistry, or concent by the instructor.)

三、參考書目(Referneces):
1. Molecular cloning: A Laboratory Manual. Joseph Sambrook - Cold Spring Harbor Laboratory Press, 2001
2. Handouts

四、課程內容(Class contents)：
1. Overview of environmental biotechnology
2. Plasmid vectors and bacterial strains
3. Extraction of plasmid DNA
4. Principle of Electrophoresis and DNA separation
5. Gel electrophoresis and DNA analysis
6. Gel electrophoresis and RNA analysis
7. Enzymes used in molecular cloning
8. Restriction enzyme digestion and fragmented DNA purification
9. Principle of gene transfer in E. coli
10. DNA ligation and transformation
11. PCR and its application
12. Detection of cloned gene by PCR
13. Principle of SDS-PAGE and protein analysis
14. SDS-PAGE
15. Expression systems for cloned genes
16. Western Blot analysis
17. Bioinformatics

五、課程要求(Evaluation)：
Final exam 50%; Term paper 50%

六、課程形式(Class format):
Class format includes lectures and lab practice.